Vessel analysis

Vessel analysis


Virtual endoscopic views of the individual hippocampal vessel of Tg-AD mice[1]



An endoscopic observation reveals the inner-surface morphology and smoothness of the vascular lumen. The color in the endoscopic images indicates the diameter of the vascular lumen. The inner surface of the vascular lumen in the Tg-AD mouse was quite rugged and were irregular in shape at the branching point. The uneven lumen surface and irregular shape of the branch-opening sites in the Tg-AD mice are likely to affect the blood flow and microcirculation in the hippocampus.





3D visualization of the glymphatic vessels in the whole mouse brain[2]




At 30 and 120 min after intracisternal infusion with fluorescent dextran (Dex-3), lectin was injected to stain the cerebral vasculature. Using fMOST, a high-resolution 3D dataset of the brain-wide distribution of Dex-3 was acquired. These cerebral regions containing large-caliber penetrating arteries and/or adjacent to the subarachnoid space had more robust CSF flow compared to other regions. Moreover, the major glymphatic vessels for CSF influx and fluid efflux in the entire brain were shown in 3D.








Typical distribution patterns of arteries and veins[3] 



Based on the brain-wide vascular spatial structures, we reconstructed and annotated the vascular system atlas of both arteries and veins of the whole mouse brain to make the precise vascular atlas (arteries are presented in red, and veins are in blue).





[1] Zhang X, Yin X, Zhang J, et al. High-resolution mapping of brain vasculature and its impairment in the hippocampus of Alzheimer's disease mice. Natl Sci Rev. 2019;6(6):1223-1238. doi:10.1093/nsr/nwz124

[2] He XZ, Li X, Li ZH, et al. High-resolution 3D demonstration of regional heterogeneity in the glymphatic system. J Cereb Blood Flow Metab. 2022;42(11):2017-2031. doi:10.1177/0271678X221109997

[3] Xiong B, Li A, Lou Y, et al. Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain. Front Neuroanat. 2017;11:128. Published 2017 Dec 19. doi:10.3389/fnana.2017.00128

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