Protein & particle distribution
Whole-brain 3D visualization of anatomical distribution of Aβ plaques
Systematic whole-brain visualization of Aβ plaque distribution is critical to characterize the key pathological features for understanding the pathology of AD. The brain-wide plaque distribution from the olfactory bulb to cerebellum was clearly visualized throughout the whole 5×FAD mouse brain.
Visualization of blood vessels surrounding Aβ plaques
We visualized the plaques and vasculatures simultaneously in the hippocampus. The distribution of plaques and vasculatures across the entire unilateral hippocampus showed that the plaque-denser areas in the ventral part exhibited a reduction of blood vessels; while the plaque-sparser areas in the dorsal part showed relatively richer blood vessels.
Distribution patterns of inhaled particles at full lung scale
Taking advantage of the fMOST system, co-localized lung-wide datasets of both cyto-architectures and fluorescent particles are collected at full scale with the resolution down to individual particles. The precise spatial distribution pattern reveals the region-specific distribution and structure-associated deposition of the inhalable particles in lungs, which is undetected by previous methods.
 Yin X, Zhang X, Zhang J, et al. High-Resolution Digital Panorama of Multiple Structures in Whole Brain of Alzheimer's Disease Mice. Front Neurosci. 2022;16:870520. Published 2022 Apr 19. doi:10.3389/fnins.2022.870520
 Sun X, Zhang X, Ren X, et al. Multiscale Co-reconstruction of Lung Architectures and Inhalable Materials Spatial Distribution. Adv Sci (Weinh). 2021;8(8):2003941. Published 2021 Feb 8. doi:10.1002/advs.202003941