Neuron morphology
Quantification of pyramidal dendritic spines and axon boutons at the single-neuron level on the brain-wide scale[1]
By using chemical sectioning fluorescence tomography (CSFT), high-throughput, high-contrast blockface imaging on multicolor fluorescent protein labeling samples can be achieved. We demonstrate mouse whole-brain imaging at the subcellular resolution, as well as the power for quantitative acquisition of synaptic-connection-related pyramidal dendritic spines and axon boutons on the brain-wide scale at the complete single-neuron level.
3D reconstruction of a pair of neighboring Purkinje cells in the ninth lobule of the cerebellum[2]
Three-dimensional reconstruction of a pair of neighboring Purkinje cells in the ninth lobule of the cerebellum. We found that the neighboring Purkinje cells are close to each other, which is different from the corresponding description in Gray’s Anatomy
Morphological analysis of the pyramidal cell-like neurons[3]
The Golgi-stained anterior cingulate cortex (ACC) neurons at the neurite level were reconstructed. The morphology and spatial locations of neuronal soma and branches could be clearly distinguished. We observed that the apical but not basal dendrites in the tree shrew and rat extended longer than that of the mouse. To confirm whether the number of branches was different, the Sholl analysis method was then processed for analyzing randomly selected layer V neurons in mouse, rat and tree shrew.
Reference
[1] Wang X, Xiong H, Liu Y, et al. Chemical sectioning fluorescence tomography: high-throughput, high-contrast, multicolor, whole-brain imaging at subcellular resolution. Cell Rep. 2021;34(5):108709. doi:10.1016/j.celrep.2021.108709
[2] Li A, Gong H, Zhang B, et al. Micro-optical sectioning tomography to obtain a high-resolution atlas of the mouse brain. Science. 2010;330(6009):1404-1408. doi:10.1126/science.1191776
[3] Lu JS, Yue F, Liu X, Chen T, Zhuo M. Characterization of the anterior cingulate cortex in adult tree shrew. Mol Pain. 2016;12:1744806916684515. doi:10.1177/1744806916684515
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