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BioMapping 1000

BioMapping 5000

BioMapping 9000

BioMapping 9500

BioMapping 1000

Comprehensive anatomy of large tissue at submicron level

Based on MOST technology, BioMapping 1000 can simultaneously visualize complex information without specific labelling. Nissl staining and Golgi staining is combined to enable the imaging to take place. This means that the instrument provides different signals with distinct grey values and morphologies. This ensures easy extraction and reconstruction of regions within the whole tissue.

Imaging mode

Brightfield high-speed linear scanning

Voxel resolution

0.35μm x 0.35μm x 1μm

Maximum sample volume

5cm x 5cm x 3cm

Slicing module

Diamond knife; 1-4μm thickness

Sample preparation

Resin embedding

Illumination

High brightness LED light

Objective lens

0.8NA water objective 40X magnification

Detection module

DALSA TDI-CCD cameras

3D High precision motion

3D precision motion system：

Travel range: 210mm@X,100mm@Y,30mm@Z

Minimum incremental motion: 0.001μm@X,Y 0.1μm@Z

Bi-directional repeatability: ±0.04μm@X,Y ±0.07μm@Z

Hardware control system

CPU 5222 3.8/3.9GHz dual-core processor

128GB DDR4 2666MHz RDIMM ECC

NVIDIA T1000

Image processing workstation

5222 3.8/3.9GHz dual-core processor

128GB or 512GB 2400MHz DDR4

NVIDIA T2000

Data storage

12x8TB RAID5 disk array，

Cache read and write speed:1GB/s

Software package

3D data visualization and analysis software

System operation software

Imaging preprocessing software

MOST technology

Ultra-thin sectioning

Automatic registration

TECHONLOGY

Whole-brain imaging of rat neuronal morphology with Golgi staining

(A) A sagittal image of a 200 µm thickness projection from a whole-brain imaging dataset acquired by MOST method . Arrowheads in different colors in (A) indicate individual neurons. (B) Pyramidal neuron in somato motor cortex (green in (A)); (C) Pyramidal neuron in anterior olfactory nucleus of olfactory bulb (blue); (D) Interneuron in glomerular layer of olfactory bulb (orange); Purkinje cell in cerebellum (brown).

Reference：

Yuan J, Gong H, Li A, Li X, Chen S, Zeng S, Luo Q. Visible rodent brain-wide networks at single-neuron resolution. Front Neuroanat. (2015);9:70. (Review)

SPECIFICATION