BioMapping 5000
BioMapping 5000
The essential brain-wide positioning system for dissecting neural structures
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Imaging mode Fluorescent high-speed linear scanning |
Voxel resolution 0.35μm x 0.35μm x 1μm |
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Acceptable wavelengths Dylight594(585nm)，mCherry(587nm)，PI(535nm)， GFP(488nm), YFP(510nm); DAPI available in triple channel |
Maximum sample volume 5cm x 5cm x 3cm |
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Slicing module Diamond knife; 1-4μm thickness |
Sample preparation Resin embedding |
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Illumination 100mW laser options Filter group （GFP/PI dual channel or DAPI/GFP/PI triple channel ） |
Objective lens 0.8NA water objective 40X magnification or 0.5NA water objective 20X magnification or 1.0NA water objective 60X magnification |
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Detection module 2 (up to 3) DALSA TDI-CCD cameras |
3D High precision motion 3D precision motion system： Travel range: 210mm@X,100mm@Y,30mm@Z Minimum incremental motion: 0.001μm@X,Y 0.1μm@Z Bi-directional repeatability: ±0.04μm@X,Y ±0.07μm@Z |
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Hardware control system CPU 5222 3.8/3.9GHz dual-core processor 128GB DDR4 2666MHz RDIMM ECC NVIDIA T1000 |
Image processing workstation 5222 3.8/3.9GHz dual-core processor 128GB or 512GB 2400MHz DDR4 NVIDIA T2000 |
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Data storage 12x8TB RAID5 disk array, Cache read and write speed:1GB/s |
Software package 3D data visualization and analysis software System operation software Imaging preprocessing software |
fMOST technology
Precise positioning
Multi-channel imaging
TECHONLOGY
Whole-brain imaging of rat neuronal morphology with Golgi staining
(A) A sagittal image of a 200 µm thickness projection from a whole-brain imaging dataset acquired by MOST method . Arrowheads in different colors in (A) indicate individual neurons. (B) Pyramidal neuron in somato motor cortex (green in (A)); (C) Pyramidal neuron in anterior olfactory nucleus of olfactory bulb (blue); (D) Interneuron in glomerular layer of olfactory bulb (orange); Purkinje cell in cerebellum (brown).
Reference：
Yuan J, Gong H, Li A, Li X, Chen S, Zeng S, Luo Q. Visible rodent brain-wide networks at single-neuron resolution. Front Neuroanat. (2015);9:70. (Review)
SPECIFICATION
|
Imaging mode Fluorescent high-speed linear scanning |
Voxel resolution 0.35μm x 0.35μm x 1μm |
|
Acceptable wavelengths Dylight594(585nm)，mCherry(587nm)，PI(535nm)， GFP(488nm), YFP(510nm); DAPI available in triple channel |
Maximum sample volume 5cm x 5cm x 3cm |
|
Slicing module Diamond knife; 1-4μm thickness |
Sample preparation Resin embedding |
|
Illumination 100mW laser options Filter group （GFP/PI dual channel or DAPI/GFP/PI triple channel ） |
Objective lens 0.8NA water objective 40X magnification or 0.5NA water objective 20X magnification or 1.0NA water objective 60X magnification |
|
Detection module 2 (up to 3) DALSA TDI-CCD cameras |
3D High precision motion 3D precision motion system： Travel range: 210mm@X,100mm@Y,30mm@Z Minimum incremental motion: 0.001μm@X,Y 0.1μm@Z Bi-directional repeatability: ±0.04μm@X,Y ±0.07μm@Z |
|
Hardware control system CPU 5222 3.8/3.9GHz dual-core processor 128GB DDR4 2666MHz RDIMM ECC NVIDIA T1000 |
Image processing workstation 5222 3.8/3.9GHz dual-core processor 128GB or 512GB 2400MHz DDR4 NVIDIA T2000 |
|
Data storage 12x8TB RAID5 disk array, Cache read and write speed:1GB/s |
Software package 3D data visualization and analysis software System operation software Imaging preprocessing software |
fMOST technology
Precise positioning
Multi-channel imaging
Address：Building G2, Future Science and Technology City, 999 Gaoxin Avenue, Donghu Hi-tech Development Zone, Wuhan, Hubei, China