BioMapping 9000
BioMapping 9000
Highly efficient workflow for rapid three-dimensional imaging
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Imaging mode Inclined illumination-detection volume scanning |
Voxel resolution 1.3μm x 1.3μm x 0.92μm |
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Acceptable wavelengths Dylight594(585nm)，mCherry(587nm)， PI(535nm)，GFP(488nm), YFP(510nm) |
Maximum sample volume 5cm x 5cm x 2.5cm |
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Slicing module Vibratome; 20-200μm thickness |
Sample preparation Agarose embedding |
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Illumination optics 100mW laser options Filter group: GFP/PI dual channel 0.3NA water objective 10X magnification |
Detection module Hamamatsu sCMOS Camera 0.5NA water objective 20X magnification |
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3D High precision motion 3D precision motion system： Travel range: 210mm@X,100mm@Y,30mm@Z Minimum incremental motion: 0.001μm@X,Y 0.1μm@Z Bi-directional repeatability: ±0.04μm@X,Y ±0.07μm@Z |
Hardware control system CPU 5222 3.8/3.9GHz dual-core processor 128GB DDR4 2666MHz RDIMM ECC NVIDIA T1000 |
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Image processing workstation 5222 3.8/3.9GHz dual-core processor 128GB or 512GB 2400MHz DDR4 NVIDIA T2000 |
Data storage 12x8TB RAID5 disk array， Cache read and write speed:1GB/s |
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Software package 3D data visualization and analysis software System operation software Imaging preprocessing software |
fMOST technology
High-throughput acqusition
Rapid sample preparation
TECHONLOGY
Case Study
We developed a micro-optical sectioning tomography (MOST) system that can provide micrometer-scale tomography of a centimeter-sized whole mouse brain.
Reference：
Yang X, Zhang Q, Huang F, et al. High-throughput light sheet tomography platform for automated fast imaging of whole mouse brain. J Biophotonics. 2018;11(9):e201800047. doi:10.1002/jbio.201800047
SPECIFICATION
|
Imaging mode Inclined illumination-detection volume scanning |
Voxel resolution 1.3μm x 1.3μm x 0.92μm |
|
Acceptable wavelengths Dylight594(585nm)，mCherry(587nm)， PI(535nm)，GFP(488nm), YFP(510nm) |
Maximum sample volume 5cm x 5cm x 2.5cm |
|
Slicing module Vibratome; 20-200μm thickness |
Sample preparation Agarose embedding |
|
Illumination optics 100mW laser options Filter group: GFP/PI dual channel 0.3NA water objective 10X magnification |
Detection module Hamamatsu sCMOS Camera 0.5NA water objective 20X magnification |
|
3D High precision motion 3D precision motion system： Travel range: 210mm@X,100mm@Y,30mm@Z Minimum incremental motion: 0.001μm@X,Y 0.1μm@Z Bi-directional repeatability: ±0.04μm@X,Y ±0.07μm@Z |
Hardware control system CPU 5222 3.8/3.9GHz dual-core processor 128GB DDR4 2666MHz RDIMM ECC NVIDIA T1000 |
|
Image processing workstation 5222 3.8/3.9GHz dual-core processor 128GB or 512GB 2400MHz DDR4 NVIDIA T2000 |
Data storage 12x8TB RAID5 disk array， Cache read and write speed:1GB/s |
|
Software package 3D data visualization and analysis software System operation software Imaging preprocessing software |
fMOST technology
High-throughput acqusition
Rapid sample preparation
Address：Building G2, Future Science and Technology City, 999 Gaoxin Avenue, Donghu Hi-tech Development Zone, Wuhan, Hubei, China